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Journal of Neurorestoratology  2017, Vol. 5 Issue (1): 117-123    doi: 10.2147/JN.S125567
Original Research     
Curcumin reduces hippocampal neuron apoptosis and JNK-3 phosphorylation in rats with Aβ-induced Alzheimer’s disease: protecting spatial learning and memory
Yun-liang Wang1,2,*, Jin-feng Li1,*, Yu-tong Wang3, Chun-yang Xu2, Lin-lin Hua2, Xiao-peng Yang2, Shuang Geng1, Shan-shan Wang1, Zhen Wang1, Hong-lei Yin1
1 Neurology Department, 148th Hospital, Zibo, Shandong;
2 Neurology Department, Second Affiliated Hospital of Zhengzhou University, Zhengzhou;
3 Public Health Department, Henan University School of Medicine, Kaifeng, Henan, People’s Republic of China
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Abstract  Amyloid-β peptide (Aβ) toxicity in Alzheimer’s disease (AD) is associated with the c-Jun N-terminal kinase (JNK) signaling pathway. Curcumin may prevent Aβ fiber formation, slowing AD progression. A model of AD was established in 32 Sprague Dawley rats by injection of 10 μg Aβ1-40 into the right hippocampus. Saline was used in sham control (n=16). Sixteen AD model rats received 300 mg/kg curcumin and another 16 received saline daily for 7 days. Spatial learning and memory were assessed using a Morris water maze. Hippocampus neuron apoptosis and hippocampal levels of JNK-3 and p-JNK-3 were assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling, reverse transcription-polymerase chain reaction and Western blotting. Aβ1-40 injection induced slower spatial learning, memory deficit, neuronal apoptosis and increased JNK-3 expression and phosphorylation (all P<0.05). Curcumin relieved spatial learning and memory deficits, hippocampus neuronal apoptosis, and reduced JNK-3 and p-JNK-3 levels (all P<0.05). In conclusion, curcumin may inhibit JNK-3 phosphorylation to protect against hippocampal neuron apoptosis after Aβ injection.

Key wordsAlzheimer's disease      curcumin      apoptosis      JNK-3      phosphorylation     
Received: 24 October 2016      Published: 26 December 2017
Corresponding Authors: Hong-lei Yin, Neurology Department, 148th Hospital, 20 Zhanbei Road, Zibo, Shandong, 255300, People’s Republic of China Tel +86 533 655 2183 Fax +86 533 655 2100 Email; *These authors contributed equally to this work   
Cite this article:

Yun-liang Wang, Jin-feng Li, Yu-tong Wang, Chun-yang Xu, Lin-lin Hua, Xiao-peng Yang, Shuang Geng, Shan-shan Wang, Zhen Wang, Hong-lei Yin. Curcumin reduces hippocampal neuron apoptosis and JNK-3 phosphorylation in rats with Aβ-induced Alzheimer’s disease: protecting spatial learning and memory. Journal of Neurorestoratology, 2017, 5: 117-123.

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