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Journal of Neurorestoratology  2017, Vol. 5 Issue (1): 133-141    doi: 10.2147/JN.S139820
Original Research     
Direct interaction of receptor tyrosine kinases, EphA4 and PDGFRβ, plays an important role in the proliferation of neural stem cells
Qingfa Chen1, Takahiro Sawada2, Kazushige Sakaguchi2, Fabin Han1,3
1 Centre for Stem Cells and Regenerative Medicine, The Institute for Tissue Engineering & Regenerative Medicine,The Liaocheng People’s Hospital/Liaocheng University, Liaocheng, People’s Republic of China;
2 Department of Molecular Cell Biology and Molecular Medicine, Institute of Advanced Medicine, Wakayama Medical University, Wakayama, Japan;
3 Centre for Stem Cells and Regenerative Medicine, The Institute of Translational Medicine, The Second Hospital of Shandong University, Jinan, Shandong, People’s Republic of China;
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Abstract  Receptor tyrosine kinases mediate the extracellular signals and transmit them into the cytoplasm by activating intracellular proteins through tyrosine phosphorylation. Both Ephs and platelet-derived growth factor (PDGF) receptors (PDGFRs) have been implicated in neurogenesis, but the functional interaction between these two pathways is poorly understood. Here, we demonstrated that EphA4 directly interacts with PDGFRβ and mutually activates each other when expressed in HEK293T cells. H9-derived neural stem cells express Ephs and PDGFRs, and their proliferation is stimulated by ephrin-A1 and PDGF-BB with further augmentation by their combined application. As both EphA4 and PDGFRβ play important roles in preventing neurodegeneration and promoting neuroprotection, their interaction and transactivation might transduce the signal through the EphA4/PDGFRβ complex and augment the proliferation of neural stem cells.

Key wordsEphA4      PDGFRβ      neural stem cell      transactivation      proliferation     
Received: 17 April 2017      Published: 26 December 2017
Corresponding Authors: Fabin Han, Center for Stem Cells and Regenerative Medicine, The Liaocheng People’s Hospital/Liaocheng University, 67 Dongchang West Road, Liaocheng, Shandong 252000, People’s Republic of China Email fhan2013@126.com; Kazushige Sakaguchi, Department of Molecular Cell Biology and Molecular Medicine, Wakayama Medical University, Kimiidera, 811-1, Wakayama, Japan Email ksaka@wakayama-med.ac.jp   
Cite this article:

Qingfa Chen, Takahiro Sawada, Kazushige Sakaguchi, Fabin Han. Direct interaction of receptor tyrosine kinases, EphA4 and PDGFRβ, plays an important role in the proliferation of neural stem cells. Journal of Neurorestoratology, 2017, 5: 133-141.

URL:

http://jnr.tsinghuajournals.com/10.2147/JN.S139820     OR     http://jnr.tsinghuajournals.com/Y2017/V5/I1/133

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