|Direct interaction of receptor tyrosine kinases, EphA4 and PDGFRβ, plays an important role in the proliferation of neural stem cells
|Qingfa Chen1, Takahiro Sawada2, Kazushige Sakaguchi2, Fabin Han1,3
|1 Centre for Stem Cells and Regenerative Medicine, The Institute for Tissue Engineering & Regenerative Medicine,The Liaocheng People’s Hospital/Liaocheng University, Liaocheng, People’s Republic of China;
2 Department of Molecular Cell Biology and Molecular Medicine, Institute of Advanced Medicine, Wakayama Medical University, Wakayama, Japan;
3 Centre for Stem Cells and Regenerative Medicine, The Institute of Translational Medicine, The Second Hospital of Shandong University, Jinan, Shandong, People’s Republic of China;
Abstract Receptor tyrosine kinases mediate the extracellular signals and transmit them into the cytoplasm by activating intracellular proteins through tyrosine phosphorylation. Both Ephs and platelet-derived growth factor (PDGF) receptors (PDGFRs) have been implicated in neurogenesis, but the functional interaction between these two pathways is poorly understood. Here, we demonstrated that EphA4 directly interacts with PDGFRβ and mutually activates each other when expressed in HEK293T cells. H9-derived neural stem cells express Ephs and PDGFRs, and their proliferation is stimulated by ephrin-A1 and PDGF-BB with further augmentation by their combined application. As both EphA4 and PDGFRβ play important roles in preventing neurodegeneration and promoting neuroprotection, their interaction and transactivation might transduce the signal through the EphA4/PDGFRβ complex and augment the proliferation of neural stem cells.
Received: 17 April 2017
Published: 26 December 2017
Fabin Han, Center for Stem Cells and Regenerative Medicine, The Liaocheng People’s Hospital/Liaocheng University, 67 Dongchang West Road, Liaocheng, Shandong 252000, People’s Republic of China Email email@example.com; Kazushige Sakaguchi, Department of Molecular Cell Biology and Molecular Medicine, Wakayama Medical University, Kimiidera, 811-1, Wakayama, Japan Email firstname.lastname@example.org
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